Plasmid

Part:BBa_K4357005:Design

Designed by: Di Yu   Group: iGEM22_UM_Macau   (2022-10-12)


J23100-XeR-mCherry-pS1C3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3774
    Illegal suffix found in sequence at 1726
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3774
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 1727
    Illegal PstI site found at 1741
    Illegal NotI site found at 1545
    Illegal NotI site found at 1734
    Illegal NotI site found at 3780
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3774
    Illegal BamHI site found at 794
    Illegal XhoI site found at 749
    Illegal XhoI site found at 1554
    Illegal XhoI site found at 2758
    Illegal XhoI site found at 3650
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3774
    Illegal suffix found in sequence at 1727
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3774
    Illegal XbaI site found at 3789
    Illegal SpeI site found at 1727
    Illegal PstI site found at 1741
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The J23100 is used to control the expression of fluoresecent signaling protein


Source

https://parts.igem.org/Part:BBa_K4357000 https://parts.igem.org/Part:BBa_B0034 https://parts.igem.org/Part:BBa_J23100 https://parts.igem.org/Part:pSB1C3 https://parts.igem.org/Part:BBa_K4357003 https://parts.igem.org/Part:BBa_B0015

References